Journal: The Journal of comparative neurology

Article Title: Immunocytochemical Organization and Sour Taste Activation in the Rostral Nuc. Solitary Tract of Mice

doi: 10.1002/cne.24059

Figure Lengend Snippet: Age and Sex of All Animals Used

Article Snippet: Rabbit polyclonal anti-P2X2 (AB_2040054) , synthetic peptide to rat C’ terminus (SQQDSTSTDPKGLAQL) , Alomone Labs, LTD Jerusaleum (APR-003) , 1:500.

Techniques:

Journal: The Journal of comparative neurology

Article Title: Immunocytochemical Organization and Sour Taste Activation in the Rostral Nuc. Solitary Tract of Mice

doi: 10.1002/cne.24059

Figure Lengend Snippet: Primary Antibodies

Article Snippet: Rabbit polyclonal anti-P2X2 (AB_2040054) , synthetic peptide to rat C’ terminus (SQQDSTSTDPKGLAQL) , Alomone Labs, LTD Jerusaleum (APR-003) , 1:500.

Techniques: Sequencing

Journal: The Journal of comparative neurology

Article Title: Immunocytochemical Organization and Sour Taste Activation in the Rostral Nuc. Solitary Tract of Mice

doi: 10.1002/cne.24059

Figure Lengend Snippet: See supplemental Fig. 1 (S1) for P2X2/ChAT immunoreactivity across all nine nTS levels. For each nTS level, a pair of images is shown, Left: photographs of P2X2 (green), ChAT (magenta), and a Nissl background stain (cyan), with nTS boundaries indicated as white dotted lines; Right: computationally extracted chartings of the image for each pixel greater than 2 S.D. of background staining. r1: In rostral sections, P2X2 immunoreactive fibers (green) label the facial nerve root as it passes medially through the dorsal medulla, terminating within the dorsocentral subnucleus of the nTS (r1 & r3). At more caudal levels (i2 – i4), P2X2 immunoreactive fibers expand medially and ventrally to terminate within the medial two-thirds of the nTS. CHAT- GFP primarily labels brainstem motor nuclei and nerve roots throughout the medulla (including DMX, 12n).

Article Snippet: Rabbit polyclonal anti-P2X2 (AB_2040054) , synthetic peptide to rat C’ terminus (SQQDSTSTDPKGLAQL) , Alomone Labs, LTD Jerusaleum (APR-003) , 1:500.

Techniques: Staining

Journal: The Journal of comparative neurology

Article Title: Immunocytochemical Organization and Sour Taste Activation in the Rostral Nuc. Solitary Tract of Mice

doi: 10.1002/cne.24059

Figure Lengend Snippet: See supplemental Fig. 2 (S2) for P2X2/NPY immunoreactivity across all nine nTS levels. In rostral nTS, NPY-immunoreactive fibers are distributed in the medial part of the nTS and within the DMX in areas that also contain ChAT-positive cells and processes. In intermediate nTS, NPY staining is densest lining the 4th ventricle (4V), but also occurs within the area postrema (AP). The nTS boundaries and organization of the figure is as described for Fig. 3.

Article Snippet: Rabbit polyclonal anti-P2X2 (AB_2040054) , synthetic peptide to rat C’ terminus (SQQDSTSTDPKGLAQL) , Alomone Labs, LTD Jerusaleum (APR-003) , 1:500.

Techniques: Staining

Journal: The Journal of comparative neurology

Article Title: Immunocytochemical Organization and Sour Taste Activation in the Rostral Nuc. Solitary Tract of Mice

doi: 10.1002/cne.24059

Figure Lengend Snippet: See supplemental Fig 3. (S3) for P2X2/galanin immunoreactivity across all nine nTS levels. Galanin+ fibers are distributed in a roughly similar pattern to that of NPY, although the peptides do not appear to co-localize within the majority of individual fibers (See Fig. 7). The nTS boundaries and organization of the figure is as described for Fig. 3.

Article Snippet: Rabbit polyclonal anti-P2X2 (AB_2040054) , synthetic peptide to rat C’ terminus (SQQDSTSTDPKGLAQL) , Alomone Labs, LTD Jerusaleum (APR-003) , 1:500.

Techniques:

Journal: The Journal of comparative neurology

Article Title: Immunocytochemical Organization and Sour Taste Activation in the Rostral Nuc. Solitary Tract of Mice

doi: 10.1002/cne.24059

Figure Lengend Snippet: See supplemental Fig. 4 (S4) for P2X2/CGRP immunoreactivity across all nine nTS levels. For each nTS level, a pair of images is shown, Left: photographs of P2X2 (green), CGRP (magenta), and a Nissl background stain (cyan), with nTS boundaries indicated as white dotted lines; Right: computationally extracted charting of the image for each pixel greater than 2 S.D. of background staining. Patterns of P2X2 immunoreactivity are similar to that described in Fig. 3 above. CGRP-immunoreactive fibers appear both within and immediately lateral to the RL subdivision of the nTS. However, a substantial number of CGRP+ fibers also terminate within the RC subdivision of nTS, overlapping the P2X2 terminal field. Moreover, a unique CGRP+ zone occurs laterally beyond the nominal boundaries of the nTS and occupies the DMSp5.

Article Snippet: Rabbit polyclonal anti-P2X2 (AB_2040054) , synthetic peptide to rat C’ terminus (SQQDSTSTDPKGLAQL) , Alomone Labs, LTD Jerusaleum (APR-003) , 1:500.

Techniques: Staining

Journal: Neuroscience Bulletin

Article Title: Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells

doi: 10.1007/s12264-019-00353-0

Figure Lengend Snippet: Protein levels of mGluR1/5 and NMDAR subunits in retinal extracts from Rac1-cKO, Chat-cre+/–, and control mice. A Representative immunoblots showing the mGluR1 and mGluR5 protein levels. B Bar charts summarizing the average densitometry of immunoreactive bands of mGluR1 and mGluR5 expression. C Representative immunoblots showing the GluN1, GluN2A, and GluN2B protein levels. D Bar charts summarizing the average densitometry of immunoreactive bands of GluN1, GluN2A, and GluN2B expression. All the data are normalized to control. n = 6–7. *P < 0.05, ***P < 0.001 vs control.

Article Snippet: After blocking in 5% non-fat milk at room temperature for 2 h, the membranes were incubated overnight at 4°C with the following primary antibodies: polyclonal mouse anti-GluN1 (1:1000; BD Pharmingen, Franklin Lakes, NJ), polyclonal rabbit anti-GluN2A (1:200; Alomone Labs, Jerusalem, Israel), polyclonal rabbit anti-GluN2B (1:200; Alomone Labs), polyclonal rabbit anti-GluA1 (1:200; Alomone Labs), monoclonal rabbit anti-mGluR1 (1:1000; Cell Signaling Technology, Danvers, MA), monoclonal rabbit anti-mGluR5 (1:500; Abcam), polyclonal rabbit anti-glycine receptor alpha1+alpha2 (1:1000; Abcam), and polyclonal rabbit anti-GABA A receptor alpha1 (GABRA1) (1:1000; Abcam).

Techniques: Western Blot, Expressing